Screening of Lignoselulolitic Fungi Origin from Tropical Forest of Indonesia

Skrining Jamur Lignoselulolitik Asal

 Hutan Tropika Indonesia

Fajar Harisma¹, Cecep Kusmana¹, Irnayuli R. Sitepu²

¹Departemen Silvikultur, Fakultas Kehutanan IPB

²Laboratorium Mikrobiologi Hutan Pusat Penelitian dan Pengembangan Hutan dan Konservasi Alam (P3HKA)

ABSTRACT

Lignoselulose is a main component of  plant, which is selulose, hemiselulose, lignin and other extractif substance. One of biomass lignoselulose benefit is bioethanol production. Bioethanol is an alcohol compound that achieve from biomass fermentation process with microorganism assist. One of microorganism that can use for ethanol production is fungi. This research aim to studying morfology of fungi that origin from tropical forest of Indonesia, studying the most potential fungi in degradated  lignin and selulose at manure media and determine the most potential fungi in degradated selulose at Carboxy Methyl Cellulose (CMC). This research divide in several stages, that is a first stage is prepare 40 fungi isolate at Potato Dextrose Agar (PDA) media, second stage is selected fungi at manure media, third stage is selected fungi at CMC media as in vitro. Based from research, it result four fungi isolate  that potential to degradated selulose at manure substrate media, that is fungi isolat FORDA CC-467, FORDA CC-468, FORDA CC-469, and FORDA CC-470. From lignin analyse result 11 isolate that potential to degradated lignin and manure substrate, that is FORDA CC-474, FORDA CC-475, FORDA CC-476, FORDA CC-477, FORDA CC-478, FORDA CC-479, FORDA CC-480, FORDA CC-481, FORDA CC-482, FORDA CC-483, and FORDA CC-484. AT CMC media, 33 isolate can degradated selulose that showed with transparent zone creation. From 33 isolate result seven isolate that have distance between micelia and transparent zone, it shows that fungi isolate is the most optimal isolate in created selulase enzim.

Kata kunci: biomassa, CMC, jamur, lignoselulolitik, skrining.