Studi
Tripsin Inhibitor Pada Pohon Sengon (Paraserianthes
falcataria (L) Nielsen) Provenan Kediri, Solomon dan Subang
Yauvina1 dan Ulfah Juniarti Siregar1
1Departemen
Silvikultur, Fakultas Kehutanan IPB
ABSTRACT
One species frequently used for establishment of forest plantation in
Indonesia is Sengon (Paraserianthes
falcataria (L.) Nielsen), because of fast growing habit and its timber
can be used for many purposes. Monoculture sengon plantation, however, greatly
suffers from stem borer, known as boktor (Xystrocera festiva Pascoe, Cerambycidae, Coleoptera), which
starts attacking 3-4 years old trees. The loss caused by the pest is quite
large. In an effort to control the pest it is important to plant resistant
trees, which can be obtained through plant breeding activities. For this
purpose it is necessary to study the presence of an antagonistic substance,
such as Trypsin inhibitors on
every part of the tree. The purpose of this study is to determine: 1) different activity
level
of trypsin inhibitors from the three provenances of Kediri, Solomon, and
Subang, 2) different activity level of trypsin
inhibitors in the bark and stem from the three provenances, 3) different activity level
of
trypsin inhibitors on healthy trees compared to heavily infestated by boktor
4). Different trypsin inhibitor activity as tested
using
synthetic and natural enzymes from the digestive gut
of boktor
larvae.
The object of
this research is sengon tree from provenances
of Kediri, Solomon, and
Subang, consisted of stem and bark, which are made into powder. Two types of tree were selected,
i.e. healthy and severely attacked trees. Observation of trypsin inhibitor activity was
carried out using the synthetic substrate benzoyl-DL-arginine-p-nitroanilid
(BAPNA), a trypsin synthetic enzyme (SIGMA 3.5 U / mg) and natural enzymes from
the digestive tract of boktor larvae, size 1.5 cm. Data were analyzed in a
Complete Randomized Factorial Design (CRFD) with three factors, i.e.
provenances, conditions of the tree, and tree tissues, using SPSS 18.0
software.
Analysis of
variance showed that provenance
factors did not significantly affect the
activity of trypsin inhibitors. Different tissues
and tree conditions, however,
have highly
significant influence on the activity of trypsin inhibitor when tested
using synthetic enzymes. Trees with healthy condition has higher
trypsin inhibitor activity than severely
attacked trees. Meanwhile the bark has higher
trypsin inhibitor activity than the stem.
Results of analysis of
variance using natural enzymes showed that the provenance and condition of the
trees had no significant effect. The results also showed that trypsin inhibitor
activity, when tested using the synthetic enzyme, is greater compared to that of
natural enzymes from the digestive tract of boktor larvae.
Keywords
: Enzim trypsin, inhibitor
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